Culturing Tetraselmis ?

[Clownfishfan]

I've cultured Nanochloropsis before and have done just fine(but after my need for it died I became a little to lax with it..so I no longer have it), this time I would like to do TET but I cannot find much info on it. I've searched the net and have only found one person that said how they culture it ,they used Kent essential elements and miracle grow. My plan was to use guilards f/2 and was wondering if that will work? The reason I ask is that the person who cultured it tried using guilards f/2 and it turned yellow until he went back to miracle grow.

[Clownfishfan]

Anyone?

[paka]

I grow TET using Micro Algae Grow and it does great. I've had it in a room with A/C at 72F and currently it's in my garage at 85F. I have it in 2 liter soda bottles and just use the regular protocol of filling the bottle, adding clorox, let sit a day or more, neutralize clorox, add nutrients, add 1ml TET and let it grow. It comes up to a dark green in about 6 days.

Hope that helps.
Karen

[Clownfishfan]

Well, well, well if it isn't the famous Karen B!
Thanks for the response ! You say you start a whole 2lt culture of TET using just a ml, it most grow faster than it though! What sg do you use,1.010?
I'm certainly going to try it within the next week,I'll post pics once I've got it going.

[paka]

To clarify, I have the cultures in 2 liter soda bottles but there is actually 1.7 liters of water in the bottle. I start my nanno and iso with 3 to 6 ml but the TET takes off with just 1 ml. I'm at a salinity around 33 to 34ppt (sorry I don't measure sg). I do get evaporation in the bottles and sometimes top them up with fresh water if I don't use the algae fast enough.

Karen

[Clownfishfan]

Parts per thousand works fine also. Wow it is fast! Your obviously doing something right

[paka]

Must be that magic Waikiki Aquarium water I'm using.

[Clownfishfan]

That's exactly what it is! I'm gonna have to use at least five times the TET than you