Our tank's biology up close - Page 14

jason2459 · 11/29/2016, 02:29 PM

Still had some "extras" even after all the rinsing but those strands I am pretty darn sure are from the vibrant.

Algae_vs_Vibrant2-0006 by Jason, on Flickr

Algae_vs_Vibrant2-0007 by Jason, on Flickr

Algae_vs_Vibrant2-0008 by Jason, on Flickr

Algae_vs_Vibrant2-0009 by Jason, on Flickr

jason2459 · 11/29/2016, 02:41 PM

Now this set was cool to watch and have it on video (hopefully) The pieces being "pulled" out of the algae.

Algae_vs_Vibrant2-0011 by Jason, on Flickr

Algae_vs_Vibrant2-0018 by Jason, on Flickr

Algae_vs_Vibrant2-0019 by Jason, on Flickr

Algae_vs_Vibrant2-0020 by Jason, on Flickr

Algae_vs_Vibrant2-0021 by Jason, on Flickr

Algae_vs_Vibrant2-0022 by Jason, on Flickr

Algae_vs_Vibrant2-0023 by Jason, on Flickr

Algae_vs_Vibrant2-0024 by Jason, on Flickr

Algae_vs_Vibrant2-0025 by Jason, on Flickr

Algae_vs_Vibrant2-0026 by Jason, on Flickr

jason2459 · 11/29/2016, 08:09 PM

Google decided to animate that sequence. I still need to get the videos uploaded.

jason2459 · 11/29/2016, 11:06 PM

Playlist
Algae with Vibrant
https://www.youtube.com/playlist?lis...D7gZ9OkfQYvwdT

And this was the cool one.

Right about the 2 minute mark I try to see where these little pieces are coming from. Then realized they were coming from the algae...

Right after the 3 minute mark I caught a nice chuck come out.

OllieNZ · 11/30/2016, 02:59 AM

Definitely an interesting result

bif24701 · 12/03/2016, 10:16 PM

Quote:

Originally Posted by jason2459

Back to Bacteria in a bottle. I've looked at Instant Ocean Biospira, Dr. Tim's one and only, and Dr. Tim's Eco Balance.

Of those it was only the one and only I didn't find any bacteria but plenty of detritus.

Tonight I'm looking at Microbacter7. It reminds me a lot of the Eco Balance but there is visually some differences in some of the bacteria.

I'm going to try this product on my reef, I have a little cyano, and very small patches of hair algae. I keep my nutrients pretty low, barely detectable if at all. I want to know if these products help with the nitrogen processing by improving the bacteria diversity. We shall see.

Sent from my iPhone using Tapatalk

gregkn73 · 12/04/2016, 07:18 AM

Until now trimming my ATS and chaeto was a sad moment, as I knew that I threw away, a lot of useful organisms. Seeing you photos make it a lot worse.

downset21 · 12/08/2016, 12:03 PM

I'm interested in the Vibrant test. Btw your work here is interesting and important!

Sent from my iPhone using Tapatalk

jason2459 · 12/08/2016, 01:06 PM

Thanks. I don't know how important it really is but it is interesting.

jason2459 · 12/13/2016, 01:02 PM

I think I've narrowed down what this smaller dinoflagellate is. I've suspected a type of Amphidinium.

From the description in this paper I'm thinking it's Amphidinium massartii. Some really cool images in this article too.
https://www.researchgate.net/publica...u_Island_Korea

From it:
Amphidinium massartii Biecheler (1952) and Amphi-
dinium carterae Hulburt (1957) are small and have some-
times been considered to be con-speciﬁc (Murray et al.
2004, 2012). However, Murray et al. (2004) suggested that
A. massartii is clearly different from A. carterae because
A. massartii plastids are centrally located and are pres-
ent at a relatively low density, while A. carterae plastids
are peripherally located and reticulated. In addition, they
also suggested that ﬂagellar insertion of A. massartii (~0.6
of the cell body from the apex) is situated in a lower po-
sition than in A. carterae (~0.4 of the cell body from the
apex).

From their toxicity testing:
Toxicity analysis
No nauplii of A. salina were dead after 48 h of incuba-
tion with A. massartii AMJJ1 concentrations of 100, 500,
1,000, 5,000, or 10,000 cells mL

Another good article with images of various dinoflagellate around Jeju island as well.
http://www.kpubs.org/article/article...2013_v36n4_347

jason2459 · 01/11/2017, 03:04 AM

I have what looks like a dusty dark dirt like area on my substrate where my last bit of cyano was during my Vibrant testing and I wanted to see what all was there.

Pics and vids uploading. I will give a little spoiler because I was very excited to find them. I finally found a couple ostracods with eggs. Very cool looking.

jason2459 · 01/11/2017, 10:24 AM

So, it mostly looked like well... dirt. It was a lot of detritus with some cyano, diatoms, sponge spicules, lots of bacteria of different types etc. Normal detritus stuff I see.

But I did find some cool stuff.

I followed this little ciliate for quite some time. It was very excited spinning very fast.

Dirt_on_Sand-0013 by Jason, on Flickr

Some cyano but not a lot

Dirt_on_Sand-0015 by Jason, on Flickr

A few Diatoms

Dirt_on_Sand-0017 by Jason, on Flickr

Dirt_on_Sand-0035 by Jason, on Flickr

Some walking ciliates

Dirt_on_Sand-0019 by Jason, on Flickr

Dirt_on_Sand-0005 by Jason, on Flickr

Sponge Spicules and here's a tight ball of them

Dirt_on_Sand-0034 by Jason, on Flickr

jason2459 · 01/11/2017, 11:08 AM

And lots of ostracods

Dirt_on_Sand-0001 by Jason, on Flickr

Dirt_on_Sand-0008 by Jason, on Flickr

Dirt_on_Sand-0018 by Jason, on Flickr

and found some that were hold eggs. This was very cool to see and see some of various sizes.

Dirt_on_Sand-0006 by Jason, on Flickr

Dirt_on_Sand-0031 by Jason, on Flickr

Dirt_on_Sand-0007 by Jason, on Flickr

Dirt_on_Sand-0010 by Jason, on Flickr

Dirt_on_Sand-0025 by Jason, on Flickr

Dirt_on_Sand-0028 by Jason, on Flickr

Dirt_on_Sand-0029 by Jason, on Flickr

jason2459 · 01/11/2017, 11:39 AM

Video Playlist
https://www.youtube.com/playlist?lis...-bCFv5KZ1Qj6ly

jason2459 · 01/23/2017, 09:31 PM

This should be pretty cool

nursedude · 01/23/2017, 10:38 PM

Wow, amazing post. I forgot how much fun it is to look through a microscope. Plus the camera allows capture. Very cool! Thank you for posting.

Sent from my iPhone using Tapatalk

jason2459 · 01/24/2017, 09:46 AM

I found the 4 they show on the label and then some. A nice bacteria count as well.

Ocean_Magik_Phytoplankton-0001 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0002 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0003 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0004 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0005 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0006 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0008 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0014 by Jason, on Flickr

Ocean_Magik_Phytoplankton-0016 by Jason, on Flickr

bertoni · 01/24/2017, 06:13 PM

Interesting. It'd be very interesting to find a way to estimate the volume of phytoplankton a a percentage, but that probably difficult, to say the least.

taricha · 02/07/2017, 06:37 AM

Hey, Jason. Have you ever run across these before?
I've seen them a couple of times but no idea what they are.
super tiny (cyano for size comparison), swim with a spiraling motion - see video 1. Pigmentation like dinoflagellate. Shaped flat like a flea.
vid 1
https://youtu.be/qFZ_Xrjo1HY

higher power vid 2
https://youtu.be/DR0qZlgSgc8

Live with cyano on sandbed - see pic

jason2459 · 02/07/2017, 07:27 AM

Quote:

Originally Posted by taricha

Hey, Jason. Have you ever run across these before?
I've seen them a couple of times but no idea what they are.
super tiny (cyano for size comparison), swim with a spiraling motion - see video 1. Pigmentation like dinoflagellate. Shaped flat like a flea.
vid 1
]

Live with cyano on sandbed - see pic

Yes, dinoflagellets for sure. Possibly a type of Amphidinium

taricha · 02/07/2017, 10:14 PM

Thanks for reply and awesome thread. I got lucky and found an ID I think I like - (movement doesn't seem a match for amphidinium dino).
Cryptomonas
and maybe more specifically a Rhodomonas
I got a better detailed video
https://youtu.be/IP0znkYW9xw
It's cool. It's a whole different class of thing than what I've ID'd in my tank before.

karimwassef · 02/07/2017, 10:38 PM

what microscope are you using to get those videos? I can't see anything that good with mine.

taricha · 02/07/2017, 11:12 PM

Quote:

Originally Posted by karimwassef

what microscope are you using to get those videos? I can't see anything that good with mine.

heh, it's a sad old high school scope (30 yr old prolly) maybe this one that was discarded by biology class - shot through galaxy 4 phone.

jason2459 · 02/07/2017, 11:30 PM

Quote:

Originally Posted by taricha

Thanks for reply and awesome thread. I got lucky and found an ID I think I like - (movement doesn't seem a match for amphidinium dino).
Cryptomonas
and maybe more specifically a Rhodomonas
I got a better detailed video
https://youtu.be/IP0znkYW9xw
It's cool. It's a whole different class of thing than what I've ID'd in my tank before.

Nice. What could help is if you could close the iris to help increase the contrast and see if you can spot two flagella.

Easier if you can capture with vid that could increase exposure and boost contrast as well. And even then it could be hard.

Looking it up even the first ID sketch of that species only had a single flagellet. Also looks like it may be one of the non-toxic species. So they would be a score minus when they bloom. lol

taricha · 02/07/2017, 11:59 PM

Quote:

Originally Posted by jason2459

Nice. What could help is if you could close the iris to help increase the contrast and see if you can spot two flagella.

Still shot of what looks like flagella coming out of the grooved end (or maybe my imagination)

And here's a few still shots stitched into animation

Sent from my SAMSUNG-SGH-I337 using Tapatalk

11/29/2016, 02:29 PM	#326
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Still had some "extras" even after all the rinsing but those strands I am pretty darn sure are from the vibrant. Algae_vs_Vibrant2-0006 by Jason, on Flickr Algae_vs_Vibrant2-0007 by Jason, on Flickr Algae_vs_Vibrant2-0008 by Jason, on Flickr Algae_vs_Vibrant2-0009 by Jason, on Flickr __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

11/29/2016, 02:41 PM	#327
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Now this set was cool to watch and have it on video (hopefully) The pieces being "pulled" out of the algae. Algae_vs_Vibrant2-0011 by Jason, on Flickr Algae_vs_Vibrant2-0018 by Jason, on Flickr Algae_vs_Vibrant2-0019 by Jason, on Flickr Algae_vs_Vibrant2-0020 by Jason, on Flickr Algae_vs_Vibrant2-0021 by Jason, on Flickr Algae_vs_Vibrant2-0022 by Jason, on Flickr Algae_vs_Vibrant2-0023 by Jason, on Flickr Algae_vs_Vibrant2-0024 by Jason, on Flickr Algae_vs_Vibrant2-0025 by Jason, on Flickr Algae_vs_Vibrant2-0026 by Jason, on Flickr __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

11/29/2016, 08:09 PM	#328
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Google decided to animate that sequence. I still need to get the videos uploaded. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

11/29/2016, 11:06 PM	#329
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Playlist Algae with Vibrant https://www.youtube.com/playlist?lis...D7gZ9OkfQYvwdT And this was the cool one. Right about the 2 minute mark I try to see where these little pieces are coming from. Then realized they were coming from the algae... Right after the 3 minute mark I caught a nice chuck come out. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

11/30/2016, 02:59 AM	#330
OllieNZ Registered Member Join Date: Aug 2010 Location: UK Posts: 569	Definitely an interesting result __________________ "Perhaps it is boredom, not intelligence, that has propelled humans up the evolutionary ladder." _________ Reefed out

12/04/2016, 07:18 AM	#332
gregkn73 Registered Member Join Date: Dec 2013 Posts: 278	Until now trimming my ATS and chaeto was a sad moment, as I knew that I threw away, a lot of useful organisms. Seeing you photos make it a lot worse.

12/08/2016, 12:03 PM	#333
downset21 Registered Member Join Date: Mar 2010 Posts: 192	I'm interested in the Vibrant test. Btw your work here is interesting and important! Sent from my iPhone using Tapatalk

12/08/2016, 01:06 PM	#334
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Thanks. I don't know how important it really is but it is interesting. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

12/13/2016, 01:02 PM	#335
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	I think I've narrowed down what this smaller dinoflagellate is. I've suspected a type of Amphidinium. From the description in this paper I'm thinking it's Amphidinium massartii. Some really cool images in this article too. https://www.researchgate.net/publica...u_Island_Korea From it: Amphidinium massartii Biecheler (1952) and Amphi- dinium carterae Hulburt (1957) are small and have some- times been considered to be con-speciﬁc (Murray et al. 2004, 2012). However, Murray et al. (2004) suggested that A. massartii is clearly different from A. carterae because A. massartii plastids are centrally located and are pres- ent at a relatively low density, while A. carterae plastids are peripherally located and reticulated. In addition, they also suggested that ﬂagellar insertion of A. massartii (~0.6 of the cell body from the apex) is situated in a lower po- sition than in A. carterae (~0.4 of the cell body from the apex). From their toxicity testing: Toxicity analysis No nauplii of A. salina were dead after 48 h of incuba- tion with A. massartii AMJJ1 concentrations of 100, 500, 1,000, 5,000, or 10,000 cells mL Another good article with images of various dinoflagellate around Jeju island as well. http://www.kpubs.org/article/article...2013_v36n4_347 __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

01/11/2017, 03:04 AM	#336
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	I have what looks like a dusty dark dirt like area on my substrate where my last bit of cyano was during my Vibrant testing and I wanted to see what all was there. Pics and vids uploading. I will give a little spoiler because I was very excited to find them. I finally found a couple ostracods with eggs. Very cool looking. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

01/11/2017, 10:24 AM	#337
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	So, it mostly looked like well... dirt. It was a lot of detritus with some cyano, diatoms, sponge spicules, lots of bacteria of different types etc. Normal detritus stuff I see. But I did find some cool stuff. I followed this little ciliate for quite some time. It was very excited spinning very fast. Dirt_on_Sand-0013 by Jason, on Flickr Some cyano but not a lot Dirt_on_Sand-0015 by Jason, on Flickr A few Diatoms Dirt_on_Sand-0017 by Jason, on Flickr Dirt_on_Sand-0035 by Jason, on Flickr Some walking ciliates Dirt_on_Sand-0019 by Jason, on Flickr Dirt_on_Sand-0005 by Jason, on Flickr Sponge Spicules and here's a tight ball of them Dirt_on_Sand-0034 by Jason, on Flickr __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

01/11/2017, 11:08 AM	#338
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	And lots of ostracods Dirt_on_Sand-0001 by Jason, on Flickr Dirt_on_Sand-0008 by Jason, on Flickr Dirt_on_Sand-0018 by Jason, on Flickr and found some that were hold eggs. This was very cool to see and see some of various sizes. Dirt_on_Sand-0006 by Jason, on Flickr Dirt_on_Sand-0031 by Jason, on Flickr Dirt_on_Sand-0007 by Jason, on Flickr Dirt_on_Sand-0010 by Jason, on Flickr Dirt_on_Sand-0025 by Jason, on Flickr Dirt_on_Sand-0028 by Jason, on Flickr Dirt_on_Sand-0029 by Jason, on Flickr __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

01/11/2017, 11:39 AM	#339
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Video Playlist https://www.youtube.com/playlist?lis...-bCFv5KZ1Qj6ly __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

01/23/2017, 09:31 PM	#340
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	This should be pretty cool __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

01/23/2017, 10:38 PM	#341
nursedude Registered Member Join Date: Aug 2011 Location: Montrose, Co Posts: 95	Wow, amazing post. I forgot how much fun it is to look through a microscope. Plus the camera allows capture. Very cool! Thank you for posting. Sent from my iPhone using Tapatalk __________________ "The problem with internet quotes is that you cant always depend on their accuracy" -Abraham Lincoln, 1864 Current Tank Info: SCAquarium 66 gallon rimless

01/24/2017, 09:46 AM	#342
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	I found the 4 they show on the label and then some. A nice bacteria count as well. Ocean_Magik_Phytoplankton-0001 by Jason, on Flickr Ocean_Magik_Phytoplankton-0002 by Jason, on Flickr Ocean_Magik_Phytoplankton-0003 by Jason, on Flickr Ocean_Magik_Phytoplankton-0004 by Jason, on Flickr Ocean_Magik_Phytoplankton-0005 by Jason, on Flickr Ocean_Magik_Phytoplankton-0006 by Jason, on Flickr Ocean_Magik_Phytoplankton-0008 by Jason, on Flickr Ocean_Magik_Phytoplankton-0014 by Jason, on Flickr Ocean_Magik_Phytoplankton-0016 by Jason, on Flickr __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

01/24/2017, 06:13 PM	#343
bertoni RC Mod Join Date: Mar 2002 Location: Mountain View, CA, USA Posts: 88,616	Interesting. It'd be very interesting to find a way to estimate the volume of phytoplankton a a percentage, but that probably difficult, to say the least. __________________ Jonathan Bertoni

02/07/2017, 10:14 PM	#346
taricha Registered Member Join Date: Mar 2010 Location: NE Miss Posts: 608	Thanks for reply and awesome thread. I got lucky and found an ID I think I like - (movement doesn't seem a match for amphidinium dino). Cryptomonas and maybe more specifically a Rhodomonas I got a better detailed video https://youtu.be/IP0znkYW9xw It's cool. It's a whole different class of thing than what I've ID'd in my tank before.

02/07/2017, 10:38 PM	#347
karimwassef Registered Member Join Date: May 2004 Location: Dallas, TX Posts: 11,033	what microscope are you using to get those videos? I can't see anything that good with mine. __________________ Failure isn't an option It's a requirement. 660g 380inwall+280smp/surge S/L/Soft/Maxima/RBTA/Clown/Chromis/Anthias/Tang/Mandarin/Jawfish/Goby/Wrasse/D'back. DIY 12' Skimmer ActuatedSurge ConcreteScape