Our tank's biology up close - Page 5

jason2459 · 04/02/2016, 09:57 AM

bat21 · 04/02/2016, 03:19 PM

Think you could get a copepod onto a slide?

Fredfish · 04/02/2016, 06:49 PM

He had already had a couple if you look closely. Not sure what page they are on though.

jason2459 · 04/02/2016, 07:15 PM

Quote:

Originally Posted by bat21

Think you could get a copepod onto a slide?

Have seen lots of various pods.

More of these on the first or second page

https://youtu.be/qJWqXQNCCTQ

jason2459 · 04/02/2016, 07:16 PM

Or how about an ostracod

[Img]20160327/dfeffe458cb2189881bfe88c70402059.jpg[/img]

https://youtu.be/zwOd5sREWGo

jason2459 · 04/02/2016, 07:16 PM

Or an isopod

https://youtu.be/9y4IgPvtvcc

https://youtu.be/6L0dqXky2fA

Tigé21v · 04/02/2016, 10:05 PM

Awesome shots! Thanks for taking the time to post them.
I was planning on getting a scope sometime in the near future. I had decided on a low power boom-style, mostly to examine incoming corals for nasties. That was before I came across this thread..
Would it be possible for you to post the magnification on some of the pics you'll be adding in the future? Or, if the magnification of the pics through the camera aren't the same as if when observing, what you estimate the magnification to be? I'm curious to know just how much power is needed to get the detail in some of your shots.

jason2459 · 04/03/2016, 07:16 PM

Quote:

Originally Posted by Tigé21v

Awesome shots! Thanks for taking the time to post them.
I was planning on getting a scope sometime in the near future. I had decided on a low power boom-style, mostly to examine incoming corals for nasties. That was before I came across this thread..
Would it be possible for you to post the magnification on some of the pics you'll be adding in the future? Or, if the magnification of the pics through the camera aren't the same as if when observing, what you estimate the magnification to be? I'm curious to know just how much power is needed to get the detail in some of your shots.

I did the first time mention the approximate magnification. I'll see what I can do the next time.

Many of the shots are with the 40x and 100x lenses. With the camera looking like around 10x on top of that or a little better. It's a little closer then using the 10x eye peices but not near as close as using the 25x eye peices.

Above is the calibration slide with the different lens x values.

Post#93
http://www.reefcentral.com/forums/sh...php?p=24440669

jason2459 · 04/03/2016, 07:41 PM

Let's see, here's some comparisons.

40x spring lens
.01mm divisions

jason2459 · 04/03/2016, 07:45 PM

100x spring and oil lens
.01mm divisions

jason2459 · 04/03/2016, 07:50 PM

That diatom is a good indicator

Here is one with a 10x lens
Full scale = 1mm

Tigé21v · 04/03/2016, 08:03 PM

Thanks so much.
Maybe I can convince the Powers That Be that I really need two scopes. I don't know if I'll be able to get the wife to go along with that or not.
This thread should be a Publc Service Reminder for everyone to remember to wash their hands after messing in their tanks....

jason2459 · 05/05/2016, 10:06 PM

What I'll be looking at tonight

Untamed Rose · 05/05/2016, 10:14 PM

Beautiful Jason!

(Comment at the first page photos...not the photo of bottles it posted after LOL )

Though am curious about the bottles, as they all seem like BS to me.

jason2459 · 05/05/2016, 11:30 PM

I have to say I'm not a fan of needing to use bacteria in a bottle or have ever used it before. But I was impressed with two out of the three here.

Dr. Tim's Eco-Balance looking pretty clear and clean

Dr.Tim's One and Only looking pretty dirty

Bio-Spira looking cloudy as well

Will work on uploading pics and vids later.

jason2459 · 05/06/2016, 08:57 PM

Dr. Tim's Eco-Balance

I was not expecting much. I found a lot. I don't know about the bottles claims but found lots of coccus shaped bacteria. Everywhere in the two small drops I placed on the slide. I only took one picture as it was all the same. And all very much alive.

jason2459 · 05/06/2016, 09:00 PM

Dr. Tim's One and Only

After seeing the Eco-Balance I was excited to look at the One and Only

First thing I noticed was how cloudy the sample was. Made me even more excited.

Then I looked into the microscope and saw what looked like a ton of detritus and was ecstatic to start zooming in looking for life.

I found some interesting things as I zoomed in

But everywhere I looked I found nothing that looked alive

I spent well over 2 hours looking for anything moving staying on certain areas for quite a long time just waiting. No videos as nothing was found to get video of.

jason2459 · 05/06/2016, 09:02 PM

Bio-Spira

Just like the Eco-Balance the sample pulled from the bottle was just as cloudy.

And looking into the scope found what looks like detritus and was disappointed not expecting much of anything again.

BUT then I zoomed in even more and quickly found LIFE.

In the video you see this little guy bobbing around on little flagelets

And found lots of bacteria and different kinds. Easier to see at 2500x but not as much in picture or video. Found various cocci and rods.

jason2459 · 05/13/2016, 09:11 PM

Back at looking at some cyano and some dinos as a bonus. Plus some cool visits with some pods.

After exploring that for a few hours I soaked up some of the excess water and dropped on it some H2O2. Awesome!

Will get pics and vids uploaded later. I'm still exploring.

jason2459 · 05/14/2016, 08:58 AM

Some pics first before adding the 3% hydrogen peroxide.

Some healthy dinos

jason2459 · 05/14/2016, 08:59 AM

They seem to like to congregate together. I followed a couple for quite some time. Video posting later

jason2459 · 05/14/2016, 09:00 AM

Cyano just falling to pieces

Dino looking like they burst

taricha · 06/02/2016, 10:37 AM

Hey. Hope you don't mind me posting a couple of vids in your thread, it seemed like the best fit. But I'd be happy to remove if you prefer. Was wondering if you'd seen anything similar?

Pruning some kenya tree, looked at a drop squeezed out from it. Lots of round brown zooxanthellae. But also surprised to find smaller, less pigmented, irregular shaped, rapidly wiggling spinning things in the sample too. They seemed to glob up and accumulate other material nearby.
I googled images of the motile phase of symbiodinium, but they have the same pigmentation and approximate size as the others. Didn't seem a match.

medium power
https://youtu.be/WunSk22PK94

higher power
https://youtu.be/bkH76hE0Bc4

I also saw the same thing from pulsing xenia sample.

jason2459 · 06/02/2016, 11:41 AM

Very nice. I have a bunch of xenia in my mantis tank I want to do that with.

NHorton · 07/13/2016, 09:12 PM

Very cool thread. Thanks for taking the time to post these.

04/02/2016, 09:57 AM	#101
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	__________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

04/02/2016, 03:19 PM	#102
bat21 Registered Member Join Date: Jan 2010 Location: Queens, NY Posts: 701	Think you could get a copepod onto a slide? __________________ --- Current Tank Info: 65g fowlr

04/02/2016, 06:49 PM	#103
Fredfish Registered Member Join Date: Oct 2000 Posts: 2,661	He had already had a couple if you look closely. Not sure what page they are on though. __________________ Advice is like a firehose. Be careful how you drink.

04/02/2016, 07:16 PM	#105
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Or how about an ostracod [Img]20160327/dfeffe458cb2189881bfe88c70402059.jpg[/img] https://youtu.be/zwOd5sREWGo __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

04/02/2016, 07:16 PM	#106
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Or an isopod https://youtu.be/9y4IgPvtvcc https://youtu.be/6L0dqXky2fA __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

04/02/2016, 10:05 PM	#107
Tigé21v Registered Member Join Date: Apr 2008 Location: St. Charles, Mo. Posts: 1,369	Awesome shots! Thanks for taking the time to post them. I was planning on getting a scope sometime in the near future. I had decided on a low power boom-style, mostly to examine incoming corals for nasties. That was before I came across this thread.. Would it be possible for you to post the magnification on some of the pics you'll be adding in the future? Or, if the magnification of the pics through the camera aren't the same as if when observing, what you estimate the magnification to be? I'm curious to know just how much power is needed to get the detail in some of your shots.

04/03/2016, 07:41 PM	#109
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Let's see, here's some comparisons. 40x spring lens .01mm divisions __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

04/03/2016, 07:45 PM	#110
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	100x spring and oil lens .01mm divisions __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

04/03/2016, 07:50 PM	#111
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	That diatom is a good indicator Here is one with a 10x lens Full scale = 1mm __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

04/03/2016, 08:03 PM	#112
Tigé21v Registered Member Join Date: Apr 2008 Location: St. Charles, Mo. Posts: 1,369	Thanks so much. Maybe I can convince the Powers That Be that I really need two scopes. I don't know if I'll be able to get the wife to go along with that or not. This thread should be a Publc Service Reminder for everyone to remember to wash their hands after messing in their tanks....

05/05/2016, 10:06 PM	#113
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	What I'll be looking at tonight __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

05/05/2016, 10:14 PM	#114
Untamed Rose Moved On Join Date: Apr 2016 Location: Eastern North Carolina Posts: 45	Beautiful Jason! (Comment at the first page photos...not the photo of bottles it posted after LOL ) Though am curious about the bottles, as they all seem like BS to me.

05/05/2016, 11:30 PM	#115
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	I have to say I'm not a fan of needing to use bacteria in a bottle or have ever used it before. But I was impressed with two out of the three here. Dr. Tim's Eco-Balance looking pretty clear and clean Dr.Tim's One and Only looking pretty dirty Bio-Spira looking cloudy as well Will work on uploading pics and vids later. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

05/06/2016, 08:57 PM	#116
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Dr. Tim's Eco-Balance I was not expecting much. I found a lot. I don't know about the bottles claims but found lots of coccus shaped bacteria. Everywhere in the two small drops I placed on the slide. I only took one picture as it was all the same. And all very much alive. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

05/06/2016, 09:00 PM	#117
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Dr. Tim's One and Only After seeing the Eco-Balance I was excited to look at the One and Only First thing I noticed was how cloudy the sample was. Made me even more excited. Then I looked into the microscope and saw what looked like a ton of detritus and was ecstatic to start zooming in looking for life. I found some interesting things as I zoomed in But everywhere I looked I found nothing that looked alive I spent well over 2 hours looking for anything moving staying on certain areas for quite a long time just waiting. No videos as nothing was found to get video of. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

05/06/2016, 09:02 PM	#118
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Bio-Spira Just like the Eco-Balance the sample pulled from the bottle was just as cloudy. And looking into the scope found what looks like detritus and was disappointed not expecting much of anything again. BUT then I zoomed in even more and quickly found LIFE. In the video you see this little guy bobbing around on little flagelets And found lots of bacteria and different kinds. Easier to see at 2500x but not as much in picture or video. Found various cocci and rods. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

05/13/2016, 09:11 PM	#119
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Back at looking at some cyano and some dinos as a bonus. Plus some cool visits with some pods. After exploring that for a few hours I soaked up some of the excess water and dropped on it some H2O2. Awesome! Will get pics and vids uploaded later. I'm still exploring. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

05/14/2016, 08:58 AM	#120
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Some pics first before adding the 3% hydrogen peroxide. Some healthy dinos __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

05/14/2016, 08:59 AM	#121
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	They seem to like to congregate together. I followed a couple for quite some time. Video posting later __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

05/14/2016, 09:00 AM	#122
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Cyano just falling to pieces Dino looking like they burst __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

06/02/2016, 10:37 AM	#123
taricha Registered Member Join Date: Mar 2010 Location: NE Miss Posts: 608	Hey. Hope you don't mind me posting a couple of vids in your thread, it seemed like the best fit. But I'd be happy to remove if you prefer. Was wondering if you'd seen anything similar? Pruning some kenya tree, looked at a drop squeezed out from it. Lots of round brown zooxanthellae. But also surprised to find smaller, less pigmented, irregular shaped, rapidly wiggling spinning things in the sample too. They seemed to glob up and accumulate other material nearby. I googled images of the motile phase of symbiodinium, but they have the same pigmentation and approximate size as the others. Didn't seem a match. medium power https://youtu.be/WunSk22PK94 higher power https://youtu.be/bkH76hE0Bc4 I also saw the same thing from pulsing xenia sample.

06/02/2016, 11:41 AM	#124
jason2459 Registered Member Join Date: Jul 2009 Location: Iowa Posts: 9,671	Very nice. I have a bunch of xenia in my mantis tank I want to do that with. __________________ rebuild and recovery log: No more red house, you'll have to click on my name and visit my homepage! You can check out my parameters at reeftronics dot net website and look for my username. Current Tank Info: 180g mixed reef w/ a beananimal overflow to a dolomite RRUGF. \| 20g long G. Smithii Mantis Tank

07/13/2016, 09:12 PM	#125
NHorton Registered Member Join Date: Jul 2012 Posts: 6	Very cool thread. Thanks for taking the time to post these.