Reef Central Online Community

Go Back   Reef Central Online Community > Marine Fish Forums > Anemones & Clownfish
Blogs FAQ Calendar

Notices

User Tag List

Reply
Thread Tools
Unread 09/28/2016, 12:13 PM   #1
jlnielsen13
Registered Member
 
jlnielsen13's Avatar
 
Join Date: Apr 2005
Posts: 599
Clownfish under a microscope !

Hello,

As a new clownfish nursery owner I have unfortunately experienced a lot of losses but as a biology teacher I thought why not learn and show my students how cool things can look under a microscope.

Here is a close-up of an 8 day old clownfish! Look at the histology of the "brain " tissue!!






NEURONS!!

Hope you enjoyed,

Jim


jlnielsen13 is offline   Reply With Quote
Unread 09/28/2016, 01:23 PM   #2
ThRoewer
Registered Member
 
ThRoewer's Avatar
 
Join Date: Jan 2015
Location: Fremont, CA
Posts: 9,555
Those aren't neurons but rather Chromatophores
Neurons require a far higher magnification and at that stage the fish would already have a fairly developed brain instead of just a few neurons.

Quote:
Originally Posted by jlnielsen13 View Post
... as a biology teacher ...
That actually scares me now...


__________________
Pairs: 4 percula, 3 P. kauderni, 3 D. excisus, 1 ea of P. diacanthus, S. splendidus, C. altivelis O. rosenblatti, D. janssi, S. yasha & a Gramma loreto trio
3 P. diacanthus. 2 C. starcki

Current Tank Info: 200 gal 4 tank system (40x28x24 + 40B + 40B sump tank + 20g refugium) + 30x18x18 mixed reef + 20g East Pacific biotop + 20g FW +...
ThRoewer is offline   Reply With Quote
Unread 09/28/2016, 02:44 PM   #3
CoralsAddiction
Registered Member
 
CoralsAddiction's Avatar
 
Join Date: Mar 2014
Location: L.A./Tahoe
Posts: 6,003
Quote:
Originally Posted by ThRoewer View Post



That actually scares me now...
I can handle a small inaccuracy if that's the case. Teacher salaries scare me a lot more.


CoralsAddiction is offline   Reply With Quote
Unread 09/28/2016, 08:33 PM   #4
ThRoewer
Registered Member
 
ThRoewer's Avatar
 
Join Date: Jan 2015
Location: Fremont, CA
Posts: 9,555
A little less money for overpriced military toys and counterproductive wars and a good chunk more for education would definitely be a great idea.
It is quite scary how the America of today is becoming reminiscent of the future in Idiocracy.


__________________
Pairs: 4 percula, 3 P. kauderni, 3 D. excisus, 1 ea of P. diacanthus, S. splendidus, C. altivelis O. rosenblatti, D. janssi, S. yasha & a Gramma loreto trio
3 P. diacanthus. 2 C. starcki

Current Tank Info: 200 gal 4 tank system (40x28x24 + 40B + 40B sump tank + 20g refugium) + 30x18x18 mixed reef + 20g East Pacific biotop + 20g FW +...
ThRoewer is offline   Reply With Quote
Unread 09/29/2016, 06:23 PM   #5
jlnielsen13
Registered Member
 
jlnielsen13's Avatar
 
Join Date: Apr 2005
Posts: 599
Wow,

Well as my friend George once said, "The jerk store called and they are out of you"

There are definitely other ways of approaching that.

I am very familiar with chromatophores, and teach about them as well. Super cool in cuttlefish.

I'll have to agree to disagree with you on the microscopes, when we teach histology we show many slides on such microscopes that show neurons in spinal tissue. They stain real nice!! I teach advanced anatomy as well so better fire me there as well.

I hope you feel better about yourself, guess they should revoke my masters, and my colleagues phd in anatomy as well as my wife's as a neurosurgery nurse practitioner who all nerded out at the "neurons".

After all, teachers aren't allowed to be wrong... we must know everything and stopped learning when we got the degree.

Thanks,

JIM


Quote:
Originally Posted by ThRoewer View Post
Those aren't neurons but rather Chromatophores
Neurons require a far higher magnification and at that stage the fish would already have a fairly developed brain instead of just a few neurons.



That actually scares me now...



jlnielsen13 is offline   Reply With Quote
Unread 09/29/2016, 07:38 PM   #6
taylor t
Registered Member
 
taylor t's Avatar
 
Join Date: Oct 2006
Location: Naperville, Il
Posts: 2,396
Jim, I appreciate your passion and sharing it with your classes. Thanks for sharing the pics. Those with eyes can see.

Luke tells me your system is really pretty cool. One of these days I'll pay you both a visit, I'd like to see it.


taylor t is offline   Reply With Quote
Unread 09/29/2016, 07:42 PM   #7
ThRoewer
Registered Member
 
ThRoewer's Avatar
 
Join Date: Jan 2015
Location: Fremont, CA
Posts: 9,555
IME teachers are wrong quite often and unfortunately react way too often precisely like you when someone points it out, especially students.
Being wrong is OK, but being thin skinned and hostile if corrected isn’t. And name calling only reflects badly on the one who does it.
Also, titles don’t prove much if anything – I’ve worked with guys who had PhDs in Mechanical Engineering, but had no clue which way to put a wrench on a nut.

That’s in the picture are not brain cells. They are not only in the wrong location but also way too scattered and way too large in proportion to the rest of the larvae’s organs:

http://www.jneurosci.org/content/26/...expansion.html
http://www.jneurosci.org/content/26/...expansion.html

This is what you see in the picture above:



__________________
Pairs: 4 percula, 3 P. kauderni, 3 D. excisus, 1 ea of P. diacanthus, S. splendidus, C. altivelis O. rosenblatti, D. janssi, S. yasha & a Gramma loreto trio
3 P. diacanthus. 2 C. starcki

Current Tank Info: 200 gal 4 tank system (40x28x24 + 40B + 40B sump tank + 20g refugium) + 30x18x18 mixed reef + 20g East Pacific biotop + 20g FW +...
ThRoewer is offline   Reply With Quote
Unread 09/29/2016, 07:43 PM   #8
mr9iron
15& Over Club
 
mr9iron's Avatar
 
Join Date: Feb 2001
Location: Missouri
Posts: 2,552
Blog Entries: 1
Thanks for sharing. I thought it was pretty cool


Sent from my iPhone using Tapatalk


__________________
Vince
______________________________

Current Tank Info: Frag tank at the moment, planning another
mr9iron is offline   Reply With Quote
Unread 09/29/2016, 08:00 PM   #9
gone fishin
Registered Member
 
gone fishin's Avatar
 
Join Date: Jul 2011
Location: Wyocena Wi
Posts: 6,936
Pretty cool pics. I can see a mouth


__________________
Tony

Current Tank Info: 180gal DT, BM NAC77 skimmer,3 Maxspect razors, Maxspect Gyre 150, 30g QT
gone fishin is offline   Reply With Quote
Unread 09/30/2016, 06:00 AM   #10
jlnielsen13
Registered Member
 
jlnielsen13's Avatar
 
Join Date: Apr 2005
Posts: 599
Unfortunately THRoewer, you failed miserably at what could have been an amazing opportunity!

Instead of teaching me about my hobby and craft, you decided to insult my intelligence, teacher salary, and then even my character. In fact you are the very reason reefcentral is dying and other sites are replacing it left and right. You are what I believe these forums call a "troll" and sadly you in the process have killed the opportunity for others to learn. Luckily on the other sites I posted we are helping each other raise baby clown fish, something I could use a lot of help with honestly!!

I should thank you though, I started class yesterday saying, " Another reefer has informed me these cells might be chromatophores" I then showed a 5 minute video on cuttlefish and it turned into another video and a 20 minute discussion! It was great. I took your failure and turned it into an amazing learning experience for 150+ high school students!! Thanks!!

My students will tell you that one of my favorite parts about being a SCIENCE teacher is that science celebrates being wrong. . We actually celebrate it in my class. We celebrate that students come into my class thinking they have blue blood in their veins. I think it is awesome that we get to correct that misconception together!

I also tell them that by the end of this class they will know 1% of what there is to know about the human body. I tell them I know maybe 5% of what there is to know, my wife after 9 years of college and 10 years being a neuro APN knows maybe 10% and the top neurosurgeons she works with only know maybe 15-20%.

My students teach me everyday and honestly they love knowing that I am learning right along with them. One actually corrected the way I pronounce Wernicke's area (in the brain) the other day! It was a great learning moment.

You are right, and I tell my students often, intelligence isn't worth much in this world! My 152 students this year get excited to come to 271 everyday because of my passion and drive for the subject, as well as my empathy towards the amazing biodiversity we have on this planet! I want them to leave not necessarily more "intelligent", but rather have a greater appreciation for our natural world and become more sustainable and educated consumers. After all, if they don't, we won't have a class for their kids called "the study of life".

Have a great day,

Jim




Quote:
Originally Posted by ThRoewer View Post
IME teachers are wrong quite often and unfortunately react way too often precisely like you when someone points it out, especially students.
Being wrong is OK, but being thin skinned and hostile if corrected isn’t. And name calling only reflects badly on the one who does it.
Also, titles don’t prove much if anything – I’ve worked with guys who had PhDs in Mechanical Engineering, but had no clue which way to put a wrench on a nut.

That’s in the picture are not brain cells. They are not only in the wrong location but also way too scattered and way too large in proportion to the rest of the larvae’s organs:

http://www.jneurosci.org/content/26/...expansion.html
http://www.jneurosci.org/content/26/...expansion.html

This is what you see in the picture above:



jlnielsen13 is offline   Reply With Quote
Unread 09/30/2016, 06:03 AM   #11
jlnielsen13
Registered Member
 
jlnielsen13's Avatar
 
Join Date: Apr 2005
Posts: 599
Thanks,

You are of course welcome anytime!! Luke has a great system as well so I think it would be worth the trip. I'll even through in a free frag from the 200+ I have growing in the basement!

Here is a video I made a year ago of my 500+ gallons running through my house to carry you over till your visit!

https://www.youtube.com/watch?v=DJV51diLq5M



Appreciate it,

Jim


Quote:
Originally Posted by taylor t View Post
Jim, I appreciate your passion and sharing it with your classes. Thanks for sharing the pics. Those with eyes can see.

Luke tells me your system is really pretty cool. One of these days I'll pay you both a visit, I'd like to see it.




Last edited by jlnielsen13; 09/30/2016 at 06:09 AM.
jlnielsen13 is offline   Reply With Quote
Unread 09/30/2016, 06:05 AM   #12
jlnielsen13
Registered Member
 
jlnielsen13's Avatar
 
Join Date: Apr 2005
Posts: 599





jlnielsen13 is offline   Reply With Quote
Unread 09/30/2016, 06:13 AM   #13
Jeff4777
Registered Member
 
Jeff4777's Avatar
 
Join Date: Sep 2015
Location: San Antonio, TX
Posts: 518
Cool pictures there, makes me want a microscope.


Jeff4777 is offline   Reply With Quote
Unread 09/30/2016, 10:27 AM   #14
Bajie
Registered Member
 
Bajie's Avatar
 
Join Date: Apr 2012
Location: Back in Blighty
Posts: 71
Hmmm ...

ThRoewer has in excess of 6500 posts
jlnielsen13 has PHD and teaches

I see a lot of knowledge which should be of benefit here.

Play nicely guys.


__________________
Soon baby ... Soon!
Bajie is offline   Reply With Quote
Unread 09/30/2016, 10:54 AM   #15
CoralsAddiction
Registered Member
 
CoralsAddiction's Avatar
 
Join Date: Mar 2014
Location: L.A./Tahoe
Posts: 6,003
Quote:
Originally Posted by CoralsAddiction View Post
I can handle a small inaccuracy if that's the case. Teacher salaries scare me a lot more.
Quote:
Originally Posted by jlnielsen13 View Post

Instead of teaching me about my hobby and craft, you decided to insult my intelligence, teacher salary, and then even my character.
Have a great day,

Jim
I was actually the one who raised the issue of teacher salary not to insult any one teacher. It was rather an expression of what happened here and around the world. Teachers are always the first ones on the blame list for even the smallest of errors but their compensation isn't always up to par with the service they provide. Luckily teaching is a passion for most and not a pursuit of excessive lifestyle. It was a much broader comment than reefing. My apologies if that insulted you.


CoralsAddiction is offline   Reply With Quote
Unread 09/30/2016, 11:55 AM   #16
jlnielsen13
Registered Member
 
jlnielsen13's Avatar
 
Join Date: Apr 2005
Posts: 599
Quote:
Originally Posted by CoralsAddiction View Post
I was actually the one who raised the issue of teacher salary not to insult any one teacher. It was rather an expression of what happened here and around the world. Teachers are always the first ones on the blame list for even the smallest of errors but their compensation isn't always up to par with the service they provide. Luckily teaching is a passion for most and not a pursuit of excessive lifestyle. It was a much broader comment than reefing. My apologies if that insulted you.
No worries,

I've never complained about my salary, although as you stated the education of my peers doesn't match the compensation as with other disciplines. You know that when you sign up for the job and as you stated, the fact that I get to share my passion for the environment and the human body with 150 new students every year is the very definition of priceless!! I wouldn't trade it for the world.

More sad that a post about clown fish went south so fast as to say it is "scary" that I am a biology teacher and also that it became a character assassination.

Unfortunately, that's how a lot of these forums go these days, especially on this site. I love reef central and wish it was what it was 5-10 years ago. People I know stay away because it quickly turns away from a learning experience to people making comments hiding behind their keyboard. You are afraid to mention your failures instead of getting help from a very educated community.

I agree, he probably has a lot of knowledge and like I said probably could have helped me, and others.

Maybe MODS should close this post as it obviously isn't helping anyone with clown fish anymore!

Thanks,

JIM


jlnielsen13 is offline   Reply With Quote
Unread 09/30/2016, 12:34 PM   #17
ThRoewer
Registered Member
 
ThRoewer's Avatar
 
Join Date: Jan 2015
Location: Fremont, CA
Posts: 9,555
There is only a learning experience when the facts are straight. If the presented facts are incorrect it turns into misinformation.

I may have made a careless remark that could be taken offensively, but you validated it by turning this into a fight and calling me names instead of just admitting that you made a mistake by calling the chromatophores "brain cells".

That was the point where it could have become a learning experience, but you rather chose to double down with insults.

I made that remark because I would hope a teacher would check first that he has the facts right before presenting them to an audience.
Furthermore I expect from a teacher to be open to critique and able to admit errors that can happen despite best efforts.
Maybe I'm expecting too much...

If someone points out that I got something wrong and provides the evidence to back it up, I can accept that. You seem to have a problem with that. That's what scares me.


__________________
Pairs: 4 percula, 3 P. kauderni, 3 D. excisus, 1 ea of P. diacanthus, S. splendidus, C. altivelis O. rosenblatti, D. janssi, S. yasha & a Gramma loreto trio
3 P. diacanthus. 2 C. starcki

Current Tank Info: 200 gal 4 tank system (40x28x24 + 40B + 40B sump tank + 20g refugium) + 30x18x18 mixed reef + 20g East Pacific biotop + 20g FW +...
ThRoewer is offline   Reply With Quote
Unread 09/30/2016, 12:37 PM   #18
Bajie
Registered Member
 
Bajie's Avatar
 
Join Date: Apr 2012
Location: Back in Blighty
Posts: 71
I for one would like to see how you progress with raising the fry and documenting here.


__________________
Soon baby ... Soon!
Bajie is offline   Reply With Quote
Unread 09/30/2016, 12:42 PM   #19
Sugar Magnolia
Registered Member
 
Sugar Magnolia's Avatar
 
Join Date: Feb 2003
Location: Northern VA
Posts: 17,691
Let's stop the personal attacks and keep this thread clean.


__________________
Adrienne

The only thing to fear is fear itself....and spiders.
Sugar Magnolia is offline   Reply With Quote
Unread 09/30/2016, 01:36 PM   #20
jlnielsen13
Registered Member
 
jlnielsen13's Avatar
 
Join Date: Apr 2005
Posts: 599
Quote:
Originally Posted by Sugar Magnolia View Post
Let's stop the personal attacks and keep this thread clean.
Please delete thread... no longer functional - Thanks Sugar Magnolia

Jim


jlnielsen13 is offline   Reply With Quote
Unread 09/30/2016, 02:05 PM   #21
Banff
Registered Member
 
Join Date: Aug 2009
Posts: 155
Ok. As a published researcher who worked specifically on neurohistochemistry and microscopy of eyes, including fish eyes, this was an interesting thread. If I had come along before TheRoewer did, I would have said something quite similar although possibly slightly less bluntly. The bluntness, I would venture to say, is just a cultural difference. I work within a very large population of international scientists and physicians and spend most of every workday in conference with representatives of each international affiliate. TheRoewer was well within the bounds of the normal everyday scientific conversations I am party to. I did not see the offense that the OP is seeing. Just a perspective from outside.


Banff is offline   Reply With Quote
Unread 09/30/2016, 02:43 PM   #22
Brieninsac
Registered Member
 
Brieninsac's Avatar
 
Join Date: Sep 2015
Posts: 782
That was a pretty mean spirited comment by ThRoewer and I expect more from a seasoned member who has offered so much in the past. ThRoewer could have corrected the OP but done so in a much more diplomatic way other then disparaging his occupation because of an oversight.


__________________
Red Sea Reefer 350 (73gal/18gal sump) / Kessil a360WE Lights
Brieninsac is offline   Reply With Quote
Unread 09/30/2016, 02:58 PM   #23
jeff240gallon
Registered Member
 
jeff240gallon's Avatar
 
Join Date: May 2016
Posts: 181
Quote:
Originally Posted by Brieninsac View Post
That was a pretty mean spirited comment by ThRoewer and I expect more from a seasoned member who has offered so much in the past. ThRoewer could have corrected the OP but done so in a much more diplomatic way other then disparaging his occupation because of an oversight.


Yeah, he also made a comment on the "counter productive war" we're fighting. I'm sure isis would agree with him (As they behead another innocent)


Sent from my iPhone using Tapatalk


jeff240gallon is offline   Reply With Quote
Unread 09/30/2016, 03:16 PM   #24
Bajie
Registered Member
 
Bajie's Avatar
 
Join Date: Apr 2012
Location: Back in Blighty
Posts: 71
Sorry but in all the clownfish threads I have read I have never seen any mention of Isis
So now this thread is well and truly fubar'd and needs to be closed


Sent from my iPhone using Tapatalk


__________________
Soon baby ... Soon!
Bajie is offline   Reply With Quote
Unread 09/30/2016, 03:17 PM   #25
ThRoewer
Registered Member
 
ThRoewer's Avatar
 
Join Date: Jan 2015
Location: Fremont, CA
Posts: 9,555
Quote:
Originally Posted by Brieninsac View Post
That was a pretty mean spirited comment by ThRoewer and I expect more from a seasoned member who has offered so much in the past. ThRoewer could have corrected the OP but done so in a much more diplomatic way other then disparaging his occupation because of an oversight.
That remark was actually supposed to be ironic, but some are so thin skinned that they take everything as an insult and overreact.

Quote:
Originally Posted by jeff240gallon View Post
Yeah, he also made a comment on the "counter productive war" we're fighting. I'm sure isis would agree with him (As they behead another innocent)


Sent from my iPhone using Tapatalk
Different war! Daesh would not exist without the war in Iraq.


__________________
Pairs: 4 percula, 3 P. kauderni, 3 D. excisus, 1 ea of P. diacanthus, S. splendidus, C. altivelis O. rosenblatti, D. janssi, S. yasha & a Gramma loreto trio
3 P. diacanthus. 2 C. starcki

Current Tank Info: 200 gal 4 tank system (40x28x24 + 40B + 40B sump tank + 20g refugium) + 30x18x18 mixed reef + 20g East Pacific biotop + 20g FW +...
ThRoewer is offline   Reply With Quote
Reply

Tags
clownfish, fry, microscope


Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On



All times are GMT -6. The time now is 05:32 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2025, Jelsoft Enterprises Ltd.
Powered by Searchlight © 2025 Axivo Inc.
Use of this web site is subject to the terms and conditions described in the user agreement.
Reef CentralTM Reef Central, LLC. Copyright ©1999-2022
User Alert System provided by Advanced User Tagging v3.3.0 (Pro) - vBulletin Mods & Addons Copyright © 2025 DragonByte Technologies Ltd.